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. 2020 Nov 22;11(4):1023–1044. doi: 10.1016/j.jcmgh.2020.11.011

Figure 3.

Figure 3

Propionate promotes IEC spreading and polarization. (A–D) MSIEs were pretreated with propionate for 16 hours and then replated onto Matrigel-coated plates for 30, 60, or 90 minutes to assess attachment and spreading. (A) Representative images of cells stained for Hoechst (blue). Scale bar = 300 μm. (B) Quantification of cell attachment with 6 samples per treatment. (C) Representative images of the cells stained for phalloidin (red) or Hoechst (blue). Scale bar = 20 μm. (D) Quantification of average cell area per sample with 6 samples per treatment. (E, F) MSIEs were pretreated with propionate for 16 hours, wounded, and stained for phalloidin (green), golgin-97 (red), and Hoechst (blue) 2 hours postwounding. (E) Representative immunofluorescent images with (∗) indicating polarized cells. Scale bar = 100 μm. (F) Quantification of cell polarization with 4 samples per treatment. ∗P < .05, ∗∗P < .01, ∗∗∗P < .001, ∗∗∗∗P < .0001 by 1-way ANOVA with Tukey’s posttest for groups of 3 or more and by Student’s t test for groups of 2.