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. 2020 Nov 22;11(4):1023–1044. doi: 10.1016/j.jcmgh.2020.11.011

Figure 5.

Figure 5

Propionate promotes IEC speed and persistence. (A–G) MSIEs were wounded and video microscopy was performed. Videos were analyzed by tracking the centroid position of 15–20 cells per sample that moved the furthest during the assay. (A) Representative phase contrast images of MSIEs at 0, 4, 8, 12, 16, and 17 hours. Scale bar = 300 μm. (B) Quantification of average migration distance with 5–6 samples per treatment. (C) Plot at origin graphs of 58 cells from 3 samples per treatment. (D) MSD of 58 cells from 3 samples per treatment. (E) Alpha values of 58 cells from 3 samples per treatment. (F) Autocorrelation of cells over time representative of 58 cells from 3 samples per treatment. (G) Average cell speed per hour of 58 cell from 3 samples per treatment. ∗∗P < .01, ∗∗∗P < .001, ∗∗∗∗P < .0001 by 1-way ANOVA with Tukey’s posttest for groups of 3 or more and by Student’s t test for groups of 2.