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. 2020 Nov 22;11(4):1023–1044. doi: 10.1016/j.jcmgh.2020.11.011

Figure 8.

Figure 8

Propionate induced migration is independent of metabolism and dependent on STAT3. (A, B) MSIEs or (C, D) jejunal enteroids monolayers were treated for 8 hours with propionate followed by a Mito Stress Test. (A, C) ECAR and (B, D) OCR over time with 3 samples per treatment for panels A and B and 4–6 samples per treatment for panels C and D. MSIEs were wounded and treated with propionate with or without (E) etomoxir or (F) DON, oligomycin, metformin, or 2-DG. Quantification of average migration distance with 3–8 samples per treatment group. (G) Quantification of average migration distance of MSIEs treated with propionate with or without HJC0152, U0126, Stattic, or rapamycin with 3 or more samples per treatment. (H) Alpha values of 45, 48, and 47 cells for control, propionate-treated, or STAT3 KO–treated samples from 3 samples per treatment. ∗P < .05, ∗∗P < .01, ∗∗∗P < .001, ∗∗∗∗P < .0001 by 1-way ANOVA with Tukey’s posttest for groups of 3 or more and by Student’s t test for groups of 2.