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. 2020 Nov 22;11(4):1023–1044. doi: 10.1016/j.jcmgh.2020.11.011

Figure 9.

Figure 9

STAT3 is critical for propionate induction of cell persistence. WT and STAT3 KO MSIEs were wounded and video microscopy was performed. Videos were analyzed by tracking the centroid position of 15–20 cells per sample that moved the furthest during the assay. (A) Representative phase contrast images of MSIEs at 0, 4, 8, 12, 16, and 17 hours. Scale bar = 300 μm. (B) Quantification of average migration distance with 4 samples per treatment. (C) Plot at origin graphs of 45, 48, and 47 cells for control, propionate-treated, or STAT3 KO–treated samples from 3 samples per treatment. (D) MSIEs were wounded and phalloidin (green) and Hoechst (blue) staining was performed 16 hours postwounding. Scale bar = 100 μm. (E) MSD of 45, 48, and 47 cells for control, propionate-treated, or STAT3 KO–treated samples from 3 samples per treatment. (F) Autocorrelation of cells over time representative of 45, 48, and 47 cells for control, propionate-treated, or STAT3 KO–treated samples from 3 samples per treatment. (G) Average cell speed per hour of 45, 48, and 47 cells for control, propionate-treated, or STAT3 KO–treated samples from 3 samples per treatment. ∗∗P < .01, ∗∗∗P < .001, ∗∗∗∗P < .0001 by 1-way ANOVA with Tukey’s posttest for groups of 3 or more and by Student’s t test for groups of 2.