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. 2021 Feb 17;17:533–543. doi: 10.2147/NDT.S293614

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© 2021 Qian et al.

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miR-216a targets RSK2. (A) The potential target genes of miR-216a predicted in several bioinformatics websites. (B) The intersection of potential target genes. (C) The expression of potential target genes in rat hippocampus examined by RT-qPCR. (D) Detection of RSK2 expression in VD rats by RT-qPCR. (E) pmirGLO plasmid mapping and sequences for RSK2-WT and RSK2-MUT. (F) The luciferase activity of WT-RSK2 and MT-RSK2 tested by dual-luciferase assay. All the experiments were repeated three times independently, and the results were averaged. Unpaired t-test was used for comparison between two groups in panel D. Two-way ANOVA was used for comparison in panels C/F. &p < 0.05, vs sham-operated rats; *p < 0.05, vs the Lv-NC; #p < 0.05, vs the NC mimic; &p < 0.05, vs sham-operated rats.