Table 1.
Sequence Used for RT-qPCR
| Gene | Forward Primer (5ʹ-3ʹ) | Reverse Primer (5ʹ-3ʹ) |
|---|---|---|
| miR-216a | TAATCTCAGCTGGCAACTGTG | GAACATGTCTGCGTATCTC |
| RSK2 | TAACCGCAGAGGTCACACTCAG | CTCAGAAACTGTGGCATCCCGA |
| FZD4 | ACTTTCACGCCGCTCATCCAGT | TCTCAGGACTGGTTCACAGCGT |
| NAA15 | TGGTCAGCCATCCATTGCTCTG | CCATCCACCTGGCAGCTTCTTT |
| RSBN1 | TGTAGGTCTCAGAGCGATGACG | GTTCACTTGTCCGAGGTAGGTAC |
| GAPDH | CATCACTGCCACCCAGAAGACTG | ATGCCAGTGAGCTTCCCGTTCAG |
| 5S | CTCGCTTCGGCAGCACAT | TTTGCGTGTCATCCTTGCG |
Abbreviations: RT-qPCR, reverse transcription quantitative polymerase chain reaction; miR, microRNA; RSK2, ribosomal S6 kinase 2; FZD4, frizzled 4; NAA15, N-alpha-acetyltransferase 15; RSBN1, round spermatid basic protein 1; GAPDH, glyceraldehyde-3-phosphate dehydrogenase.