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. 2021 Feb 17;14:1117–1129. doi: 10.2147/OTT.S289241

Figure 3.

Figure 3

CircDNM3OS acted as a sponge for miR-145-5p in CCA cells. (A) Schematic diagram of circDNM3OS sequence complementary to miR-145-5p. (B) Analysis of the expression of miR-145-5p in HuCCT1 and HuH28 cells transfected with miR-145-5p mimic or miR-NC by qRT-PCR. (C, D) The luciferase activity in HuCCT1 and HuH28 cells was determined by dual-luciferase reporter assay. (E, F) The enrichment of miR-145-5p and circDNM3OS in Anti-AGO2 complex and Anti-IgG complex was detected by qRT-PCR. (G, H) Relative expression of miR-145-5p in CCA tissues and cells (HuCCT1 and HuH28) was tested using qRT-PCR. (I) The correlation between circDNM3OS and miR-145-5p expression in CCA tissues was determined by Pearson’s correlation analysis. (J) Relative expression of miR-145-5p in HuCCT1 and HuH28 transfected with in-miR-NC or in-miR-145-5p was evaluated by qRT-PCR. (K) HuCCT1 and HuH28 were transfected with si-NC, si-circDNM3OS, si-circDNM3OS+in-miR-NC, or si-circDNM3OS+in-miR-145-5p. Relative expression of miR-145-5p in HuCCT1 and HuH28 cells was detected using qRT-PCR. *P < 0.05.