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. 2021 Feb 17;14:1117–1129. doi: 10.2147/OTT.S289241

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© 2021 Su et al.

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CircDNM3OS regulated malignancy and glutamine metabolism of CCA cells through sponging miR-145-5p. (A–I) HuCCT1 and HuH28 cells were co-transfected with si-NC or si-circDNM3OS and in-miR-NC or in-miR-145-5p. (A–F) Assessment of the proliferation, colony formation, migration, invasion, apoptosis of HuCCT1 and HuH28 cells using CCK-8 assay (A, B), colony formation assay (C), transwell assay (D), wound-healing assay (E), or flow cytometry assay (F). (G–I) The levels of glutamine, α-KG, and ATP in HuCCT1 and HuH28 cells were measured using commercial kits. *P < 0.05.