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. 2021 Feb 17;14:1117–1129. doi: 10.2147/OTT.S289241

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© 2021 Su et al.

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MiR-145-5p targeted MORC2 to modulate malignancy and glutamine metabolism of CCA cells. (A–I) HuCCT1 and HuH28 cells were transfected with miR-NC, miR-145-5p, miR-145-5p+pcDNA, or miR-145-5p+MORC2. (A–F) Assessment of the proliferation, colony formation, migration, invasion, and apoptosis of HuCCT1 and HuH28 cells by CCK-8 assay (A, B), colony formation assay (C), transwell assay (D), wound-healing assay (E), or flow cytometry assay (F). (G–I) Detection of the levels of glutamine, α-KG, and ATP in HuCCT1 and HuH28 cells using commercial kits. *P < 0.05.