Figure 6.

CircHIPK3 regulates SIRT1 by interacting with miR-326/miR-487a-3p in HG-induced HK-2 cells. (A) Protein expression levels of SIRT1 in HK-2 cells by siRNA SIRT1 was measured using Western blot. (B) CCK-8 assay was used to measure the cell viability of HK-2 cells after vector transfection, transfection with circHIPK3, circHIPK3 co-transfected with miR-326 mimics or miR-487a-3p mimics and circHIPK3 transfection cooperated with silencing SIRT1. (C, D) Colony formation assays and Western blot were used to measure the apoptosis and the apoptotic proteins of HK-2 cells after vector transfection, transfection with circHIPK3, circHIPK3 co-transfected with miR-326 mimics or miR-487a-3p mimics and circHIPK3 transfection cooperated with silencing SIRT1. **p < 0.01, ^##p < 0.01, ^{^^}p < 0.01.