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. 2021 Jan 21;35(2):e21331. doi: 10.1096/fj.202001973R

FIGURE 1.

FIGURE 1

S mansoni soluble egg antigens improve metabolic homeostasis in obese mice by a STAT6‐dependent mechanism. WT and Stat6 −/− mice were fed a LFD (white bars) or a HFD for 12 weeks and next received intraperitoneal injections of PBS (black bars) or 50 µg S mansoni soluble egg antigens (SEA; red bars) every 3 days for 4 weeks (A). At sacrifice, epididymal WAT was collected and SVF was isolated and analyzed by flow cytometry. The complete gating strategy is shown in Figure S1. Frequencies of IL‐5 and IL‐13‐expressing Th2 cells (B) in WAT were determined after PMA/ionomycin/Brefeldin A restimulation. Abundances of eosinophils (C) and YM1+ macrophages (AAMs; D) were determined. Body weight (E) was measured after 4 weeks of treatment. Food intake (F) was monitored throughout the experimental period. HOMA‐IR (G) was calculated using fasting blood glucose and plasma insulin levels at week 4. An i.p. glucose tolerance test was performed at week 3. Blood glucose levels were measured at the indicated time points (H‐I) and the AUC of the glucose excursion curve was calculated (J). Data shown are a pool of two independent experiments. Results are expressed as means ± SEM. *P < .05 vs HFD, # P < .05 vs WT (n = 9‐12 mice per group)