FIGURE 5.

pLe^X‐ω1 inhibits fasting‐induced refeeding and improves metabolic homeostasis through inhibition of food intake in obese mice. Mice were fed a HFD for 12 weeks and fasted overnight prior to intraperitoneal injections of either PBS (black bars) or 50 µg pLe^X‐ω1 (green bars; A). Food intake (B) and body weight changes (C) were next monitored during 24 hours after refeeding. Mice were fed a HFD for 12 weeks, single‐housed, and next received biweekly intraperitoneal injections of PBS or 50 µg pLe^X‐ω1 during 4 weeks. In one group (H + pair‐fed; orange bars), the amount of food available for PBS‐treated mice was adjusted daily in order to match the food intake of the pLe^X‐ω1‐treated group (D). At the end of the experiment, eWAT was collected, processed, and analyzed as described in the legend of Figure 1. The frequencies of IL‐13‐expressing CD4 T cells (E), eosinophils (F), and YM1⁺ macrophages (G) were determined. Body weight change (H) was determined after 4 weeks. HOMA‐IR (I) was calculated at week 4 and an i.p. glucose tolerance test (J and K) was performed at week 3, as described in the legend of Figure 1. Results are expressed as means ± SEM. *P < .05 vs HFD or as indicated (n = 3‐5 mice per group)