FIGURE 6.

The metabolic effects of pLe^X‐ω1 are independent of leptin signaling in hyperphagic obese mice. Seven weeks old obese db/db mice received biweekly intraperitoneal injections of PBS (white bars) or 50 µg pLe^X‐ω1 (green bars) during 4 weeks (A). At the end of the experiment, eWAT was collected, processed, and analyzed as described in the legend of Figure 1. The frequencies of IL‐13‐expressing CD4 T cells (B), eosinophils (C), and YM1⁺ macrophages (D) were determined. Body weight (E) and food intake (G) were monitored throughout the experimental period, and body composition (F) was measured after 4 weeks. HOMA‐IR (H) was calculated at week 4 and intraperitoneal glucose (I and J) and insulin (K and L) tolerance tests were performed as described in the legend of Figures 1 and 3. Results are expressed as means ± SEM. *P < .05 vs PBS (n = 5‐6 mice per group)