FIGURE 1.

The effects of postmortem delay (PMD) on viable fluorescence‐activated cell sorting (FACS)‐sorted mouse microglia numbers and RNA quality. (a) FACS plots depicting the sorting strategy of DAPI^neg Ly6C^neg CD11b^high CD45^int microglia and the PMD induced decrease in the percentage of DAPI^neg microglia in gate R5. (b) Dot plot depicting the number of DAPI^neg microglia sorted from entire mouse brains at different PMD times (n = 4 mice per PMD). (c) Dot plot depicting RIN values of RNA extracted from sorted DAPI^neg Ly6C^neg CD11b^high CD45^int microglia at different PMD times (n = 4 mice per PMD). A one‐way ANOVA followed by a Bonferroni correction for multiple comparisons was performed to assess significance. **, p < .01; ***, p < .001