Skip to main content
. 2020 Dec 9;27(10):3315–3325. doi: 10.1002/chem.202003140

Figure 6.

Figure 6

Amination of benzyl alcohol (1 a) to benzylamine (1 c) using a single dual‐activity ADH/AmDH variant for both oxidation and reductive amination steps. wild‐type LysEDH and all of the LE‐AmDH variants were tested for this transformation. Cascade 1 (one‐enzyme cascade) buffer: Tris‐HCl (100 mm) supplemented with NH4OH (1 m) final pH 9; T: 30 °C; reaction time: 48 h; agitation orbital shaker (170 rpm); [1 a]: 10 mm; [NAD+]: 1 mm; [LysEDH or LE‐AmDH variant]: 90 μm. Cascade 2 (one LE‐AmDH variant plus NOx and FDH for orthogonal NAD cofactor recycling) buffer: Tris‐HCl (100 mm) supplemented with NH4OH (1 m), final pH 9; T: 30 °C; reaction time: 48 h; agitation orbital shaker (170 rpm); [1 a]: 10 mm; [NAD+]: 1 mm; [NOx]: 10 μm; [LysEDH or LE‐AmDH variant]: 90 μm; added after 24 hours: [HCOONa]: 100 mm and [FDH]: 16 μm.