Table 3.
Reduction of aromatic aldehydes to alcohols by LE‐AmDH variants.[a]
|
% Analytical yield of alcohol[b] | ||||||||
|---|---|---|---|---|---|---|---|---|
|
Sub. |
WT |
v1 |
v22 |
v24 |
v25 |
v27 |
NC1 |
NC2 |
|
1 b |
82 |
76 |
54 |
66 |
56 |
77 |
7 |
n.d. |
|
2 b |
74 |
64 |
59 |
53 |
46 |
80 |
5 |
n.d. |
|
3 b |
83 |
85 |
80 |
94 |
79 |
86 |
18 |
n.d. |
|
4 b |
11 |
11 |
7 |
9 |
7 |
40 |
1 |
n.d. |
|
5 b |
84 |
65 |
24 |
59 |
21 |
>99 |
n.d. |
n.d. |
|
6 b |
59 |
52 |
28 |
51 |
33 |
>99 |
1 |
n.d. |
|
7 b |
n.d. |
n.d. |
n.d. |
n.d. |
n.d. |
n.d. |
n.d. |
n.d. |
|
8 b |
n.d. |
n.d. |
n.d. |
n.d. |
n.d. |
4 |
n.d. |
n.d. |
|
9 b |
67 |
68 |
68 |
67 |
68 |
67 |
61 |
n.d. |
[a] Experimental conditions: 1 mL final volume in Eppendorf tubes; buffer: KPi (100 mm, pH 7.0); T: 30 °C; reaction time: 24 h; agitation orbital shaker (170 rpm); [substrate]: 20 mm; [NAD+]: 1 mm; [LysEDH or LE‐AmDH variant]: 45 μm; [Cb FDH]: 16 μm. NC 1: reaction without LysEDH or LE‐AmDH variant; NC 2: reaction without any enzyme addition (LysEDH or LE‐AmDH, and FDH). [b] The analytical yields reported here are the average values obtained from four independent experiments. n.d.: not detected.