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Cells lacking the polarity factor Rsr1 form multiple buds upon starvation but spore buds are able to penetrate the ascus wall. rsr1∆/rsr1∆ strain MMY0291 was cultured in (a) sporulation medium, (b) rich medium for 1 week or (c) sporulation medium, followed by pulse labelling with calcofluor white, and then rich medium to induce germination. Fluorescence images show calcofluor fluorescence
