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. 2020 Dec 10;60(6):3131–3137. doi: 10.1002/anie.202011793

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© 2020 The Authors. Angewandte Chemie International Edition published by Wiley-VCH GmbH

This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc-nd/4.0/ License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made.

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Cryo‐EM analysis of Ca_v1.1 bound separately to RBK, SBK, and amlodipine in nanodiscs. A) Chemical structures of (S)‐(−)‐Bay K8644 (SBK), (R)‐(+)‐Bay K8644 (RBK), and levamlodipine (amlodipine). B) Last step purification for rCa_v1.1 in MSP2N2‐surrounded nanodiscs. Shown here is a representative size‐exclusion chromatogram (SEC) and Coomassie blue‐stained SDS‐PAGE. C) Local resolution maps and densities for the bound ligands. Left: Resolution heatmap for the overall 3DEM reconstruction of rCa_v1.1‐100S. The unit for the scale bar is Å. Right: The local resolutions for the bound ligands. The density of the ligand in each reconstruction is highlighted with an orange square in the upper row. The densities in the lower row, shown as blue mesh, are contoured at 3σ and prepared in PyMol.