Figure 1.

Selection against Agrobacterium tumefaciens.

(a) Chemical structures of cephaloridine, fucidin and cetrimide (CFC).

(b) Pseudomonas syringae grows on CFC selection, Agrobacterium does not. Nicotiana benthamiana leaves were infiltrated with 1 × 10⁶ CFU ml⁻¹ P. syringae or 1 × 10⁸ CFU ml⁻¹ AtumGV3101, and bacterial populations were determined 3 days later using colony count method using Luria−Bertani (LB) plates containing CFC or not. Error bars represent SE of n = 3 biological replicates. Student’s t‐test statistics (***P < 0.001). CFU, colony‐forming units.