Figure 3.

PAMP receptors reduce bacterial growth in the agromonas assay.

(a) Transient expression of tomato FLS3 and Arabidopsis EFR in Nicotiana benthamiana confers flgII‐28 and elf18 responsiveness, respectively. Leaf discs from agroinfiltrated leaves expressing FLS3 (blue), EFR (red) or empty vector (EV; grey) were treated with 100 nm flgII‐28 or elf18, and reactive oxygen species (ROS) was measured in relative light units (RLU). Error intervals (shaded regions) represent SE of n = 12 biological replicates.

(b) Transient expression of FLS3 or EFR reduces Pseudomonas syringae growth. Two days after agroinfiltration, agroinfiltrated leaves expressing FLS3 (blue), EFR (red) or EV (grey) were spray‐inoculated with the indicated strains of P. syringae (at 1 × 10⁸ CFU ml⁻¹), and bacterial growth was measured 3 days later using cephaloridine, fucidin and cetrimide (CFC) selection. Error bars represent SE of n = 3 biological replicates. Student’s t‐test statistics (*P < 0.05).

(c) Transient expression of EFR, but not FLS3, affect Agrobacterium growth. Bacterial growth of AtumGV3101 was measured by plating the leaf extracts described in (b) on medium containing gentamicin. Error bars represent SE of n = 3 replicates. Student’s t‐test statistics (^* P < 0.05).