Figure 5.

Rapid functional analysis of immune components.

(a) Time scale for functional analysis by generation transgenic plants and by agroinfiltration (agromonas assay).

(b) Phosphomutant EFR^Y836F is unable to trigger reactive oxygen species (ROS) burst upon elf18 treatment. Leaves were agroinfiltrated with EFR (red), EFR^Y836F (light red) or empty vector (EV; grey), and the ROS burst was measured at 3 dpi in leaf discs treated with 100 nm elf18. Error intervals represent SE of n = 12 replicates.

(c) Phosphomutant EFR^Y836F is blocked in elf18‐triggered immunity. Two days after agroinfiltration, agroinfiltrated leaves expressing EFR, EFR^Y836F or EV were spray‐inoculated with 1 × 10⁸ CFU ml⁻¹ PtoDC3000(∆hQ) and bacterial growth was measured 3 days later using cephaloridine, fucidin and cetrimide (CFC) selection. Error bars represent SE of n = 3 biological replicates. Student’s t‐test statistics (*P < 0.05).

(d) Agroinfiltration of EFR, but not EFR^Y836F, reduces Agrobacterium growth. Bacterial growth of AtumGV3101 was measured by plating the leaf extracts described in (c) on medium containing gentamicin. Error bars represent SE of n = 3 biological replicates. Student’s t‐test statistics (^** P < 0.01).