Figure 7.

β‐Galactosidases reduce bacterial growth of BGAL‐sensitive strains in agromonas assay.

(a) NbBGAL1 and AtBGAL8 have β‐galactosidase activity. FDG‐hydrolysing activity was measured in apoplastic fluids isolated from bgal1 mutant leaves transiently expressing NbBGAL1 or AtBGAL8. Error bars represent SE of n = 3 biological replicates.

(b) Agroinfiltration of NbBGAL1 and AtBGAL8 reduce Pta6605 growth. Two days after agroinfiltration, agroinfiltrated leaves expressing NbBGAL1 or AtBGAL8 were spray‐inoculated with 1 × 10⁸ CFU ml⁻¹ Pta6605 and bacterial growth was measured 3 days later using cephaloridine, fucidin and cetrimide (CFC) selection. Error bars represent SE of n = 6 biological replicates; t‐test P‐values (^* P < 0.05).

(c) NbBGAL1 or AtBGAL8 do not reduce PsyB728a growth. Two days after agroinfiltration, agroinfiltrated leaves expressing NbBGAL1 or AtBGAL8 were spray‐inoculated with 1 × 10⁸ CFU ml⁻¹ PsyB728a and bacterial growth was measured 3 days later using CFC selection. Error bars represent SE of n = 3 biological replicates; t‐test P‐values.

(d) NbBGAL1 and AtBGAL8 do not affect Agrobacterium growth. Bacterial growth of AtumGV3101 was measured by plating the leaf extracts described in (c) on medium containing gentamicin. Error bars represent SE of n = 3 biological replicates. Student’s t‐test statistics.