FIGURE 6.

YAP/TAZ are required for remyelination. (a) Schematic showing experimental procedures analyzing control and Yap ^cHet; Taz ^cKO sciatic nerves, 20 and 60 days after sciatic nerve crush injury (n. crush). Tamoxifen (tx) was injected 5 days after nerve crush injury. (b–e) Semithin sections of the distal sciatic nerve at 20 (b) and 60 days (c) after sciatic nerve crush injury. At 20 and 60 days after injury, Yap ^cHet; Taz ^cKO sciatic nerves show a severe delay in remyelination, with most axons being unmyelinated axons (d,e). (f,g) G ratio versus axon diameter scatter plot graphs for control and Yap ^cHet; Taz ^cKO sciatic nerves at 20 and 60 days after injury. At 20 days after injury all myelinated axons were counted for Yap ^cHet; Taz ^cKO. Bars, 10 mm. n ≥ 3 mice for each genotype and time point. Data are presented as means ± SEM. Two‐sided Student's t test: ****, p ≤ 0.0001; **, p ≤ .01. No statistical analysis was performed on (f) [Color figure can be viewed at wileyonlinelibrary.com]