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. 2021 Feb 19;38(5):655–664. doi: 10.1089/neu.2020.7226

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© Ayman H. Faroqi et al., 2020; Published by Mary Ann Liebert, Inc.

This Open Access article is distributed under the terms of the Creative Commons Attribution Noncommercial License (http://creativecommons.org/licenses/by-nc/4.0/) which permits any noncommercial use, distribution, and reproduction in any medium, provided the original author(s) and the source are credited.

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FIG. 1. — (A) Schematic of plasma-membrane–bound pmeLUC construct (B). The enzymatic domain of luciferase is exposed to the extracellular space. Luciferase catalyzes the conversion of D-Luciferin to a photon of light in the presence of ATP. (C) Sagittal mouse brain section immunostained with an anti-firefly luciferase antibody reveals widespread CNS expression of pmeLUC 1 month after injections of pAAV9-CBA-pmeLUC-WPRE into mouse pups. (D, E) Magnified inserts from (C) show pmeLUC expression throughout the hippocampus and cortex. (F) Higher magnification of pmeLUC-positive neurons in the cortex reveals restricted plasma membrane. (G) Control animal injected with pAAV9-CBA-FLuc-WPRE (FLuc) reveals expression throughout the entire neuron, including the nucleus (black arrows). Scale bars, C = 2 mm; D,E = 300 μm; F = 50 μm; G = 100 μm. ADP, adenosine diphosphate; ATP, adenosine triphosphate; CNS, central nervous system; GPI, glycosylphosphatidylinositol.