Fig. 5.

Variations in cytoskeletal proteins in high-density fractions. A) The relative abundance of cytoskeletal proteins in HDFs detected by high-resolution MS/MS analysis is represented as z-score. Significant reduction of cytoskeletal proteins was seen in rpAD-specific HDFs (F12–F17: HDF pool 12 to 17). B) Differences in expression of cytoskeletal and associative proteins as determined by the SWATH-MS. Heatmaps represent the relative protein expression indicated as z-scores for spAD (n = 3), rpAD (n = 3), DLB (n = 3), rDLB (n = 2), D-FTL (n = 3), SVD (n = 3), and controls (n = 3). C) Model showing the recruitment of G2L2 to high-density prion-oligomers. The recruitment of G2L2 towards HDPs results in the loss of its binding to EB-1, affecting the actin-guided microtubule (MT) integrity