Table 2.
Effects of siRNA knockdowns of Polλ and other TLS Pols on replicative bypass of a cis-syn TT dimer or a (6-4) TT photoproduct carried on the leading DNA strand template in XPV HFs or in WT or Polλ−/− MEFs.
| Cells | UV lesion | siRNA | Number of Kan+ colonies | Number of blue colonies among Kan+ | TLS (%)a |
|---|---|---|---|---|---|
| XPV HFs | cis-syn TT dimer | NC | 312 | 34 | 10.9 |
| Rev3 | 308 | 17 | 5.5 | ||
| Rev7 | 321 | 18 | 5.6 | ||
| Polλ | 274 | 16 | 5.8 | ||
| Rev3 + Polλ | 332 | 19 | 5.7 | ||
| Rev7 + Polλ | 294 | 16 | 5.4 | ||
| XPV HFs | (6-4) TT PP | NC | 405 | 62 | 15.3 |
| Polθ | 294 | 23 | 7.8 | ||
| Rev3 | 348 | 25 | 7.2 | ||
| Polλ | 355 | 26 | 7.3 | ||
| Rev3 + Polλ | 304 | 25 | 8.2 | ||
| Polθ + Polλ | 278 | 10 | 3.6 | ||
| WT MEFs | cis-syn TT dimer | NC | 376 | 120 | 31.9 |
| Polλ−/− MEFs | NC | 432 | 104 | 24.1 | |
| Rev3 | 295 | 70 | 23.7 | ||
| Polη | 248 | 27 | 10.9 | ||
| WT MEFs | (6-4) TT PP | NC | 422 | 128 | 30.3 |
| Polλ−/− MEFs | NC | 390 | 57 | 14.6 | |
| Rev3 | 276 | 42 | 15.2 | ||
| Rev1 | 388 | 10 | 2.6 | ||
| Polθ | 408 | 11 | 2.7 |
a
Statistical analyses of these data are shown in Table S2.