Table 3.
Effects of catalytically active (WT) Polλ or catalytically inactive D427A and D429A mutant Polλ on TLS opposite a cis-syn TT dimer, a (6-4) TT photoproduct, a thymine glycol, or a 1,N6-ethenodeoxyadenosine, carried on the leading strand template in Polλ−/− MEFs.
| DNA lesion | Vector expressing | Number of Kan+ colonies | Number of blue colonies among Kan+ colonies | TLS (%)a |
|---|---|---|---|---|
| cis-syn TT dimer | No Polλ (control) | 355 | 87 | 24.5 |
| WT Polλ | 348 | 111 | 31.9 | |
| Mutant Polλ | 409 | 124 | 30.3 | |
| (6-4) TT PP | No Polλ (control) | 308 | 46 | 14.9 |
| WT Polλ | 316 | 95 | 30.1 | |
| Mutant Polλ | 286 | 40 | 14.0 | |
| Tg | No Polλ (control) | 446 | 46 | 10.3 |
| WT Polλ | 395 | 84 | 21.3 | |
| Mutant Polλ | 361 | 80 | 22.2 | |
| εdA | No Polλ (control) | 311 | 34 | 10.9 |
| WT Polλ | 405 | 82 | 20.2 | |
| Mutant Polλ | 286 | 62 | 21.7 |
a
Statistical analyses of these data are shown in Table S3.