Table 5.
UV-induced mutation frequencies in the cII gene in BBMEF cells expressing a (6-4) PP photolyase, cyclobutane pyrimidine dimer (CPD) photolyase, or no photolyase and treated with siRNA for Polλ or other TLS Pols.
| Photolyase | siRNA | UVa | Photoreactivationb | Mutation frequencyc (×10−5) |
|---|---|---|---|---|
| (6-4) PP photolyase | NC | − | + | 16.1 ± 0.7 |
| Polλ | − | + | 16.8 ± 0.7 | |
| NC | + | + | 45.9 ± 1.9 | |
| Polη | + | + | 88.7 ± 1.7 | |
| Polθ | + | + | 17.5 ± 1.1 | |
| Polλ | + | + | 30.1 ± 1.0 | |
| Rev3 | + | + | 31.5 ± 1.6 | |
| Rev1 | + | + | 58.2 ± 2.7 | |
| Rev3 + Polλ | + | + | 31.8 ± 1.1 | |
| Rev1 + Polλ | + | + | 20.1 ± 1.5 | |
| CPD photolyase | NC | − | + | 17.9 ± 1.2 |
| Polλ | − | + | 19.1 ± 1.1 | |
| NC | + | + | 28.9 ± 1.0 | |
| Rev1 | + | + | 18.4 ± 1.6 | |
| Polθ | + | + | 18.4 ± 1.2 | |
| Polλ | + | + | 40.9 ± 1.2 | |
| Rev3 | + | + | 39.1 ± 2.4 | |
| Rev3 + Polλ | + | + | 39.6 ± 1.2 | |
| Rev1 + Polλ | + | + | 20.4 ± 1.6 | |
| Polθ + Polλ | + | + | 21.8 ± 1.4 | |
| pNeo vector (no photolyase) | NC | − | + | 14.8 ± 1.0 |
| Polλ | − | + | 15.0 ± 1.3 | |
| NC | + | + | 55.5 ± 1.9 | |
| Polλ | + | + | 35.0 ± 1.0 | |
| Rev3 | + | + | 36.8 ± 2.9 |
a
5 J/m2 of UVC (254 nm) light.
b
Photoreactivation with UVA (360 nm) light for 3 h.
c
Data are represented as mean ± SEM. Mean mutation frequencies and standard error of the mean were calculated from four independent experiments.