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. 2020 Dec 17;19(2):547–561. doi: 10.1111/jth.15171

FIGURE 6.

FIGURE 6

Synergistic effects of the multimerin 1 (MMRN1)‐binding GPAGPOGPX peptides and other collagen mimetic peptides on human platelet adhesion. Panels show adhesion of human platelets pre‐activated with 10 µg/mL collagen‐related peptide (CRP; to pre‐expose platelet MMRN1 and von Willebrand factor [VWF] and activate platelet integrins), unless otherwise indicated. Data are shown as mean absorbance ± standard error of the mean, for three different samples/group, each tested in triplicate to obtain a mean for each sample, evaluated with the indicated combination of peptides or with GPP (negative control) and Horm collagen (positive control). A, Platelet adhesion to all peptides except GFOGER was indistinguishable from background binding to GPP. B, The VWF‐binding sequence III‐23 and GPAGPOGPX peptides enhanced platelet adhesion to GFOGER but not to each other. C, Co‐presentation of III‐23 and GPAGPOGPX peptides increased platelet adhesion to GFOGER. D, Effect of platelet activation with 10 µg/mL CRP on platelet adhesion to GFOGER and GFOGER co‐presented with either GPAGPOGPX or III‐23