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. 2021 Feb 3;4(4):e202000858. doi: 10.26508/lsa.202000858

Figure S2. Expression of GLUT1 and GLUT3 chimera in Xenopus oocytes.

Figure S2.

(A) Western blot analysis between GLUT1-injected oocytes, GLUT3 chimera C1-injected oocytes, and water-injected oocytes, from total membrane and plasma membrane preparations. β-integrin is used as a plasma membrane loading control and calnexin as an ER control. (B) Michaelis–Menten analysis of 2-DG uptake between GLUT1 and GLUT3 chimera C1 in Xenopus oocytes. The data were fitted using the Michaelis–Menten non-linear fit, yielding a Km = 8.6 ± 0.8 mM and Vmax = 4,920 ± 155 pmol/oocyte/30 min for GLUT1 and Km = 3.3 ± 0.5 mM and Vmax = 2,537 ± 99 pmol/oocyte/30 min for GLUT3-C1. Data represent the mean ± SD of three or more replicate experiments.