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. 2021 Jan 21;4(3):e202000918. doi: 10.26508/lsa.202000918

Figure 2. The GET complex is required for ER targeting of non-imported mitochondrial carrier proteins.

Figure 2.

(A) Super-resolution images of wild-type or get1/2Δ mutant cells expressing Oac1-GFP and Tom70-mCherry −/+ FCCP. White arrow marks perinuclear ER. White arrowhead marks protein foci containing Oac1-GFP. Images show single focal plane. (B, C) Quantification of (A) showing the percentage of cells with Oac1-GFP localized to the ER (B) or protein foci (C). N > 100 cells per replicate, error bars = SEM of three replicates. (D) Super-resolution images of wild-type or get1/2Δ cells expressing Oac1-GFP and Get3-mCherry −/+ FCCP. White arrow marks perinuclear ER. White arrowhead marks protein foci containing Oac1-GFP. Yellow arrowheads mark protein foci containing Get3-mCherry. Images show single focal plane. (E) Quantification of (D) showing the number of foci only containing Oac1-GFP (green), Get3-mCherry (magenta), or colocalized/associated Oac1-GFP and Get3-mCherry (yellow) per 100 cells −/+ FCCP. N > 100 cells per replicate of three replicates, values are normalized to number of foci per 100 cells. (F) Western blot probing for GFP and FLAG in input or elution products of immunoprecipitated Get3-GFP in the indicated yeast strains. Scale bar = 2 μm. See also Figs S3–S5.