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. 2021 Jan 21;4(3):e202000918. doi: 10.26508/lsa.202000918

Figure S2. Newly synthesized non-imported mitochondrial proteins are targeted to the ER (related to Fig 1).

Figure S2.

(A, B) Wide-field images of yeast expressing Alo1-GFP (A) or Oac1-GFP (B) and Tom70-mCherry −/+ FCCP −/+ CHX (cycloheximide). White arrow marks perinuclear ER. (C, D) Wide-field images of indirect immunofluorescence staining against the FLAG epitope in yeast expressing Alo1- or Oac1-FLAG and Tom70-mCherry (C) or Sec61-mCherry (D) −/+ FCCP. Nucleus stained with NucBlue. White arrow marks perinuclear ER. Images show single focal plane. Scale bar = 2 μm.