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. 2021 Feb 22;19:21. doi: 10.1186/s12964-021-00708-z

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 5 — FZD5 signaling requires its co-receptor LRP5. a A heat map showing the correlation of LRP5 with FZD5 and EMT-related factors in gastric cancer cell lines was generated from CCLE database. b LRP5 expression in MKN45 cells was detected by Western blot and Real-time PCR. **P < 0.01, ***P < 0.001, vs shCtrl. c LRP5 and ELF3 expression was detected by Western blot in MKN45 cells. d ZEB1 mRNA level in MKN45 cells was detected by Real-time PCR. ***P < 0.001, vs shCtrl. e Migration of MKN45 cells was analyzed by Transwell. ***P < 0.001, vs shCtrl. Scalebar: 50 μm. f MKN45 cells were stained with Phalloidin. Scalebar, 50 μm. g LRP5 and ELF3 expression in HGC27 cells was detected by Western blot. h ZEB1 mRNA level in HGC27 cells was detected by Real-time PCR. ***P < 0.001, vs OE Ctrl; ##P < 0.01, vs OE FZD5. i Migration of HGC27 cells was analyzed by Transwell. ***P < 0.001, vs OE Ctrl; ###P < 0.001, vs OE FZD5. Scalebar: 50 μm. All experiments were performed in triplicate