Extended Data Fig. 2. Juvenile social isolation leads to long lasting reduction of sociability in adult mice.

(A) Timeline showing weaning at p21 and subsequent 2 weeks of juvenile social isolation (jSI), followed by re-housing or control group housing (GH). (B) jSI mice showed reduced sociability scores vs GH mice in a 3 chamber task, in which a mouse chooses between a social target and an object, and time spent investigating both is measured and compared (two tailed t-test, t₃₆=2.154, *P=0.038, n=20 biologically independent GH mice, n=18 biologically independent jSI mice) and reduced social interaction (two-way RM ANOVA, housing (GH/jSI) × stimulus (social/object) interaction F_1,36 = 7.042, *P=0.012, effect of housing F_1,36 = 1.117, P=0.298, effect of stimulus F_1,36 = 14.860, P=0.460×10⁻³, n=20 biologically independent GH mice, n=18 biologically independent jSI mice) (C) jSI mice showed no difference in distance traveled during the open field test (two tailed t-test, t₃₆=0.939, P=0.354, n=20 biologically independent GH mice, n=18 biologically independent jSI mice), suggesting normal motor activity. While jSI mice showed reduce time in center during open field test (two tailed t-test, t₃₆=2.054, *P=0.047, n=20 biologically independent GH mice, n=18 biologically independent jSI mice), they showed no difference in an independent anxiety task (elevated plus maze (EPM: two tailed t-test, t₃₈=0.926, P=0.360, n=20 biologically independent GH mice, n=18 biologically independent jSI mice). Data in B, C are presented as mean +/− s.e.m.