Figure 3.

Synthesis of RNA‐PROTACs and their mechanism of action. A) Synthetic route to RNA‐PROTACs and controls. B) Fluorescence polarization assay for ORN3P₁ and negative seq‐control ORN5P₁. C,D) RNA‐PROTAC‐mediated ubiquitination of myc‐Lin28A in 22Rv1 cells co‐transfected with HA‐Ubiquitin (HA‐Ub) and pCMV‐myc‐Lin28A, or pDEST‐Myc‐QKI with either vehicle (OptiMEM) or 500 nM ORN3P₁ (C) or ORN7P₁ (D). cMyc‐immunoprecipitated lysates were separated by SDS‐PAGE followed by western blots detecting HA (Ub). Slow‐migrating smears in right‐hand lanes represent ubiquitin‐conjugated cMyc‐Lin28A. WCL=whole‐cell lysate input.