Figure 3.

Gene expression changes due to genome editing of enhancers. Genomic regions corresponding to enhancer regions 1 and 4 were targeted for deletion in SW948 cells using CRISPR/Cas9 technology. Pools of transfected cells were analyzed using qPCR and Taqman gene expression assays for GREM1, FMN1, and GUSB (control). Targeting of both regions 1 and 4 enhancers resulted in a decrease in GREM1 expression levels, while FMN1 expression levels did not change significantly. qPCR, quantitative polymerase chain reaction