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. 2021 Feb 6;73(1):303–317. doi: 10.1002/hep.31259

Fig. 3.

Fig. 3

Replication stress induced by Tg E2F7/8 expression leads to DNA damage and apoptosis. (A) Representative pictures of γ‐H2AX immunohistochemistry of small intestines (top) and livers (bottom) of Tg mice after 6 days of doxycycline treatment. Scale bars, 50 μm (top) and 20 μm (bottom). (B) Quantification of total γ‐H2AX‐positive nuclei in 10 fields using ×40 objective of small intestine and liver sections after 3 and 6 days of doxycycline treatment. Crossbars represent average (n = 5 mice). (C) Quantification of total cleaved caspase 3–positive nuclei in 10 fields using ×40 objective of small intestine sections after 3 and 6 days of doxycycline treatment. Crossbars represent average (n = 5 mice). Data information: In (B,C), *P < 0.05, **P < 0.01, ***P < 0.001 (Kruskal‐Wallis one‐way analysis of variance on ranks and Dunnett’s method for multiple comparisons versus control). Abbreviations: hpf, high‐power field; n.s., not significant.