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. 2021 Feb 6;73(1):303–317. doi: 10.1002/hep.31259

Fig. 5.

Fig. 5

Antiproliferative effects of E2F7/8 overexpression cause the appearance of liver tumor nodules lacking transgene expression. (A) Representative anti‐EGFP immunohistochemistry pictures showing E2f7‐EGFP Tg expression in livers treated for 1 month with doxycycline. Scale bars, 200 μm (main), 50 μm (side pictures). Dashed lines delineate tumor nodules. (B) Proposed model of the observed and described variation in expression and proliferative capacity of cells overexpressing E2F7 both in cell culture system and in mice. Dark green and yellow nuclei represent high and low E2F7‐EGFP expression levels, respectively. (C) Percentage of immunohistochemically stained EGFP‐negative nodules relative to the total number of microscopic tumors in the indicated genotype. Bars represents average per genotype. *P < 0.05 (t test). (D) Dot plot represents the count of EGFP‐positive hepatocyte nuclei in five fields (×40 objective) per nodule. At least three random tumor nodules were analyzed per animal, n = at least 10 mice/genotype). **P < 0.01, ***P < 0.001 (Mann‐Whitney rank sum test).