Skip to main content
. 2021 Feb 6;73(1):303–317. doi: 10.1002/hep.31259

Fig. 6.

Fig. 6

Selection pressure by inducible overexpression of E2F7 in vitro favors proliferation of cells with low levels of E2F7‐EGFP. (A) Quantitative PCR results showing EGFP transcript expression in inducible E2F7‐EGFP HeLa cells and EGFP after 1, 2, 8, 12, and 20 days of doxycycline administration. Two independent experiments. (B) Proliferation curves of HeLa cells with inducible E2F7‐EGFP and EGFP after doxycycline treatment. Colored lines represent average ± SD of two technical replicates for each time point. Representative curves of two independent experiments. (C) Experimental scheme of FACS experiment to determine E2F7‐EGFP overexpression levels on clonogenic capacity of HeLa cells. (D) Representative images of colony formation assay with cells sorted in (C) from two independent clones. Two independent experiments were performed in duplicate for three different cell clones of inducible E2F7‐EGFP HeLa cell lines. Abbreviations: Dox, doxycycline; veh, vehicle.