Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2020 Dec 4;253(3):315–325. doi: 10.1002/path.5589

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2020 The Authors. The Journal of Pathology published by John Wiley & Sons, Ltd. on behalf of The Pathological Society of Great Britain and Ireland.

This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

PMC Copyright notice

Figure 3. — Anatomical variations in dermal ECM. (A) RNA expression (FPKM) of differentially expressed ECM genes with back‐ (top row) and cheek‐ (bottom row) specific expression patterns. Plotted points are skin/wound isolations from four mice per anatomical site. *padj < 0.05. (B) The ratio of soluble:insoluble collagen and (C) total collagen determined using Sircol assays on 4 mm biopsy punches of skin from different anatomical sites. Plotted points represent paired (i.e. mouse‐matched) independent skin isolations from two or three mice. (D) Histological analyses (H&E, top row) and second harmonic generation imaging (middle row) of dermis illustrate site‐specific characteristics (e.g. bundle thickness, density, orientation) of the collagenous ECM. Scale (H&E): 200 μm; (second harmonic): 50 μm. (Bottom row) Representative examples of maximum projection confocal images of cell‐derived matrices (immunostained for fibronectin) from fibroblasts established from dermis of different anatomical sites. Scale: 50 μm. (E) HVG histology illustrates differing tissue responses to damage, particularly the contribution of collagenous matrix (bright pink with HVG stain) from the fascia deep to the panniculus carnosus muscle (M). Scale bars: 100 μm. Arrowheads (filled): watershed between normal dermis and the wound bed; arrows (open heads) illustrate the contribution of tissue layers to the wound bed. (F). RNA expression (FPKM) of Engrailed (En)‐1. Bars/error bars = mean ± SD. Abbreviations and colour‐coding: A, abdomen (lateral plate mesoderm‐derived; green); B, back (paraxial mesoderm‐derived; orange); C, cheek (craniofacial, neural crest‐derived; blue).