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. 2020 Apr 6;117(3):792–804. doi: 10.1093/cvr/cvaa089

Figure 1.

Figure 1

Pregnancy increased co-localization of RyR1/RyR2 with BKCa channel in uterine arteries. (A) Representative confocal immunofluorescence images from five replicates show the co-localization of RyRs and BKCa channels in uterine arteries. Uterine arteries of non-pregnant (NUA) and pregnant (PUA) animals were stained with antibodies against the β1 subunit of BKCa channel (BKCa β1, red) and RyR1, RyR2, or RyR3 (green). Merged images show the co-localization of RyR1 or RyR2 with BKCa β1 (in yellow). The nuclear region was stained with DAPI and shows in blue. Scale bar: 100 µm. (B) PLA assay to confirm the co-localization of RyR1/RyR2 and BKCa β1 in uterine arteries. (C) Quantification of the PLA signals. Images from five independent replicates were analysed. The nuclear region was stained with DAPI and shown in blue. Scale bar: 50 µm. Data are means ± SEM from five animals of each group; independent-samples t-test; *P < 0.05, PUA vs. NUA.