Figure 2.

Mitochondrial fission inhibitor-1 mdivi1 decreased AAA formation induced by AngII plus BAPN. About 8–10-week-old C57BL/6J male mice were treated with AngII (1 μg/kg/min subcutaneous infusion) for 4 weeks with BAPN in drinking water (0.625 g/L) for the first 2 weeks (AII+B) with mdivi1 (mdiv) injections (25 mg/kg i.p. 3 times/week) or vehicle (vehi) injections (10 μL DMSO plus 90 μL corn oil i.p. 3 times/week) for 4 weeks. Control mice were sham-operated. (A) Representative photographs of the mouse aortas were demonstrated. Scale bar is 3 mm. Maximum external diameter/D of the aortas was determined in the aorta picture after aortic isolation and cleaning, sham n = 6, vehi AII+B n = 16, mdiv AII+B n = 12. Data are expressed as mean±SEM. Statistical significance was determined by one-way ANOVA with Bonferroni post hoc test. ****P < 0.0001. (B) Maximal aortic internal diameter/D was determined by echo analysis using high-resolution 2D imaging (B mode) with high-frequency ultrasound (VisualSonics Vevo2100) on Days 0 (0 W), 14 (2 W), 21 (3 W), and 28 (4 W) of the study, sham n = 5, vehi AII+B n = 12, mdiv AII+B n = 10. Data are expressed as mean±SEM. Statistical significance was determined by two-way ANOVA with Bonferroni post hoc test. *P < 0.05, ***P < 0.001, ****P < 0.0001. (C) Histological assessment was done by Masson’s Trichrome staining following euthanasia of animals at 28 days, dissection of abdominal aorta and paraffin embedding and sectioning (5 μm thick), n = 4. Scale bar; 200 μm (40×), 50 μm (200×).