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Aspartate 179 and aspartate 180 are indispensable for RyR2 N-terminus tetramerization. Chemical cross-linking assays (n = 8) of NTWT, NTK167A + Q168A, NTE173A + K174A, and NTD179A + D180A as described in the legend to Figure 1. Data are given as mean value ± SEM; statistical analysis was carried out using Kruskal–Wallis test with Dunn’s multiple comparison test.
