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. Author manuscript; available in PMC: 2021 Apr 1.
Published in final edited form as: Cancer Res. 2020 Jul 6;80(19):4071–4086. doi: 10.1158/0008-5472.CAN-19-2269

Figure 6. Aqp7 knockdown leads to changes in urea and arginine metabolism.

Figure 6.

(A) (Left) Metabolomics of urea/arginine metabolism with decreased Aqp7 expression. Cell colors are described in Figure 4. (Right) RT-qPCR of arginine metabolism genes. (B) Nitric oxide levels following Aqp7 KD in 4T1 and EpH4 cells. (C) Western analysis of arginine metabolism genes after AQP7 KD in 4T1 and EpH4 cells. (D) Urea levels in serum and urine in mice injected with 4T1 Scr. Control or Aqp7 KD cells. (E–F) Proliferation of 4T1 Aqp7 KD cells and scrambled control cells in arginine-free media. (G) (Left) H&E and (Right) MALDI-MS images of arginine and citrulline in 4T1 tumors.