(A) Western analysis of indicated proteins in 4T1 and EpH4 cells. (B) Model of AQP7 function during breast cancer and metabolome reprogramming. Aberrant AQP7 expression alters lipid metabolism, carbohydrate metabolism, nucleotide synthesis, and urea/arginine metabolism. With high intracellular glycerol or active AQP7, cells increase p38 signaling, NO, and oxidative tolerance. With low intracellular glycerol conditions or decreased AQP7 expression, cells increase phospho-mTOR signaling, making them potentially more susceptible to mTOR inhibitors.