Table 1 -.
Technologies for High-Throughput Single Cell Sequencing
| Method | Technology | Name | Platform | Cost | Chemistry | References | |
|---|---|---|---|---|---|---|---|
| scRNA-seq | microdroplets | 10X Genomics RNA | commercial | 10K | $$$$ | 3' or 5' | Zheng et al. 2017 |
| microdroplets | Drop-seq | research | 10K | $$ | 3' | Macosko et al. 2015 | |
| microdroplets | Indrop | research | 10K | $$ | 3' | Klein et al. 2015 | |
| nanowells | Seq-Well | research | 10K | $ | 3' | Gierahn et al. 2017 | |
| nanowells | Takara Wafergen | commercial | 1.8K | $$$$ | 3', full-length | Goldstein et al. 2017 | |
| nanowells | cytoseq | research | 100K | $$ | 3' | Fodor et al. 2015 | |
| FACS | smart-seq2 | research | 384 | $ | full-length | Ramskold et al. 2012 | |
| FACS | sci-RNA-seq | research | 100K | $$ | 3' | Cao et al. 2017 | |
| scEpigenomics | microdroplets | 10X Chromium ATAC | commercial | 10K | $$$$ | tagmentation | Satpathy et al. 2019 |
| FACS | ATAC tagmentation | research | 384 | $ | tagmentation | Buenrostro et al. 2015 | |
| FACS | dscATAC-seq | research | 100K | $ | tagmentation | Cusavonich et al. 2015 | |
| micromanipulation | scRBBS | research | 100 | $$ | RBBS | Guo et al. 2013 | |
| scDNA-seq | microdroplets | 10X Chromium CNV | commercial | 10K | $$$$ | MDA | Andor et al. 2018 |
| microdroplets | Mission Bio Tapestri | commercial | 10K | $$$ | amplicon PCR | Lan et al. 2017 | |
| nanowells | Wafergen Takara | commercial/res | 1.8K | $$$$ | tagmentation | Laks et al. 2019 | |
| Microfluidics | tagmentation | research | 200 | $$ | tagmentation | Zahn et al. 2017 | |
| FACS | sci-seq | research | 100K | $$ | tagmentation | Adey et al. 2017 | |