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. Author manuscript; available in PMC: 2021 Feb 22.
Published in final edited form as: Antiviral Res. 2018 Jul 25;158:13–24. doi: 10.1016/j.antiviral.2018.07.015

Fig. 5. A HSF1 positive feedback loop amplifies PI3K/Akt/mTOR signaling within HCMV-infected monocytes.

Fig. 5.

(A-B) Monocytes were mock or HCMV infected, or treated with GM-CSF or M-CSF for (A) 30 m or (B) 24 h. (C-D) Monocytes were pretreated with LY294002, MK, or rapamycin for 1 h, after which cells were either mock or HCMV infected for (C) 30 m or (D) 24 h. (A-D) HSF1 and p-HSF1 expression were determined by immunoblotting. (E-F) Prior to 24 h mock or HCMV infection, monocytes were pretreated with KRIBB11 (a HSF1 inhibitor) for 1 h. (E) mTOR and p-mTOR or (F) HSF1, p-HSF1, Mcl-1, HSP-27, and XIAP expression were assessed by immunoblot. (A-F) Membranes were then reprobed for β-actin as a loading control. Data are representative of 3–5 independent blood donors.