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. 2021 Feb 18;13:1673–1686. doi: 10.2147/CMAR.S295833

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© 2021 Wei et al.

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Downregulation of miR-138-5p reversed the effect of circ_0006174 on proliferation, migration, invasion and apoptosis of CRC cells. (A) The expression of miR-138-5p in 40 CRC tissues and adjacent normal tissues was detected by RT-qPCR. (B) The relationship between miR-138-5p expression and circ_0006174 expression was assessed by Pearson’s correlation analysis. (C) MiR-138-5p expression in CRC cells and the normal colon epithelial cells was detected by RT-qPCR. (D) The effect of miR-138-5p inhibitor on miR-138-5p expression in CRC cells was assessed by RT-qPCR. (E and F) CCK-8 assay was conducted to assess the viability of CRC cells transfected with sh-circ #1 + anti-miR-138-5p, sh-circ#1 + anti-NC or sh-NC + anti-NC. (G) The proliferation of CRC cells transfected with sh-circ #1 + anti-miR-138-5p or negative controls was measured by clone formation assay. (H and I) The migration and invasion abilities of CRC cells transfected with sh-circ #1 + anti-miR-138-5p or negative controls were estimated by transwell assay. (J) The apoptosis of CRC cells transfected with sh-circ #1 + anti-miR-138-5p or negative controls was detected by flow cytometry assay. Three independent assays were performed, and data were presented as means ± SD. *P<0.05.