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. 2020 Dec 24;12(1):654–665. doi: 10.1364/BOE.405869

Fig. 4.

Fig. 4.

True one to one comparison of PARS and bright-field images of hematoxylin and eosin (H&E) stained human skin tissue with basal cell carcinoma (BCC). a) (1) 5x bright field image of tissue with BCC demonstrating the border of invasive cancer (bottom of red border) versus normal tissue (top of red border). (2) PARS image of the same unstained sample with BCC, the same red border denotes the cancer boundary. Scale Bar: 200 µm b) (1) 20x bright field image demonstrating the same cancer margin as in (a.1, a.2). (2) Enlarged section (green box) of the PARS image (a.2) compares the disorganized cellular architecture seen in the light microscopy (b.1) and PARS images (the same red border separates cancerous and normal tissues). Scale Bar: 100 µm c) (1) 20x bright field of tissue with clearly identifiable atypical nuclear morphology, size and distribution. (2) Enlarged section (red box) of the PARS image (b.2), providing a near perfect match to the brightfield histology image (c.1). Scale Bar: 40 µm. (Dynamic range presented in decibels).