Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2021 Feb 22;10:e57417. doi: 10.7554/eLife.57417

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2021, Zhou et al

This article is distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use and redistribution provided that the original author and source are credited.

PMC Copyright notice

Figure 5. — (A) Expression of 14 parameters as shown in an unpaired principle component analysis of Tregs purified and stimulated with αCD3ε and all cytokine conditions for 3 days unless otherwise noted in the vectors. N = 3. (B–F) Surface expression of receptor subunits that comprise the multiple forms of the IL-2R and IL-4 on purified Tregs from Foxp3^RFP/Il10^GFP dual reporter mice that were stimulated for 3 days with or without αCD3ε and with all cytokine combinations. Expression of receptor subunits is plotted as a function of IL-10 expression. N = 3. (G and H) Quantification of IL-10 expression and protein concentration secreted by Tregs that were purified and cultured for 3 days with αCD3ε and all combinations of IL-2, IL-4, and IL-13, as analyzed by flow cytometry and ELISA, respectively. N = 3. (I) Surface expression of IL-10R on purified Tregs from Foxp3^RFP/Il10^GFP dual reporter mice that were stimulated for 3 days with or without αCD3ε and with all cytokine combinations. Expression of receptor subunits is plotted as a function of IL-10 expression. N = 3. (J) IL-10 expression of purified Tregs stimulated with αCD3ε and all cytokine conditions for 3 days with or without neutralizing IL-10R monoclonal antibody or isotype control blockade, as analyzed by flow cytometry (see also Figure 5—figure supplement 1). N = 3. For all panels, mean ± SEM are indicated. *p<0.05.

Figure 5—figure supplement 1. — (A) Expression of 14 parameters as shown in an unpaired principle component analysis of Tregs purified and stimulated with αCD3ε and all cytokine conditions for 3 days unless otherwise noted in the vectors. N = 3. (B–F) Surface expression of receptor subunits that comprise the multiple forms of the IL-2R and IL-4 on purified Tregs from Foxp3^RFP/Il10^GFP dual reporter mice that were stimulated for 3 days with or without αCD3ε and with all cytokine combinations. Expression of receptor subunits is plotted as a function of IL-10 expression. N = 3. (G and H) Quantification of IL-10 expression and protein concentration secreted by Tregs that were purified and cultured for 3 days with αCD3ε and all combinations of IL-2, IL-4, and IL-13, as analyzed by flow cytometry and ELISA, respectively. N = 3. (I) Surface expression of IL-10R on purified Tregs from Foxp3^RFP/Il10^GFP dual reporter mice that were stimulated for 3 days with or without αCD3ε and with all cytokine combinations. Expression of receptor subunits is plotted as a function of IL-10 expression. N = 3. (J) IL-10 expression of purified Tregs stimulated with αCD3ε and all cytokine conditions for 3 days with or without neutralizing IL-10R monoclonal antibody or isotype control blockade, as analyzed by flow cytometry (see also Figure 5—figure supplement 1). N = 3. For all panels, mean ± SEM are indicated. *p<0.05.